Product Overview

GenXflo: Everything You Need to Build Smarter Bioinformatics Pipelines

GenXflo is a next-generation, no-code bioinformatics workflow builder that empowers scientists to design, validate, and export fully functional data analysis pipelines — all through an intuitive visual interface.

Built for researchers who want to focus on science instead of syntax, GenXflo transforms the complex process of pipeline creation into a simple, four-step journey: Drag → Connect → Validate → Export.

Whether you're running RNA-seq, variant calling, or genome annotation workflows, GenXflo helps you go from idea to executable workflow in minutes, no programming required.

Step 1

Drag

Step 2

Connect

Step 3

Validate with AI

Step 4

Export

Start by dragging your favorite bioinformatics tools onto the workflow canvas.

• FastQC for quality control
• FastP and BBduk for read trimming
• HISAT2, STAR, and Bowtie2 for alignment
• Samtools for file manipulation
• FeatureCounts, Salmon, and StringTie for quantification
• FreeBayes for variant calling
• Dedup for marking duplicates
• SPAdes and Prokka for assembly and annotation

Each tool is represented as a visual block (node) on the canvas. You can add as many as you like to create single-step analyses or complex, multi-stage pipelines.

Dragging tools is simple and intuitive, just click, drag, and drop. No terminal commands, no syntax errors, no setup headaches.

Next, connect your tools in the order they should run.

In GenXflo, the connectors (arrows) between nodes define how data flows from one step to another. For example, the output of FastQC can feed into FastP, whose output then moves into HISAT2 for alignment.

The connections are biologically meaningful and logically validated, ensuring that output file types match the input requirements of the next tool.

This visual linking allows you to:

• Build complete RNA-seq, variant calling, or metagenomic workflows.
• Clearly see the structure of your analysis.
• Reorder or rewire your workflow at any time with a simple drag.

The canvas updates dynamically as you design, helping you visualize your pipeline from raw data to final output in real time.

In GenXflo, your analysis literally takes shape in front of your eyes.

GenXflo's AI validation system works behind the scenes in real time to detect and prevent common issues during workflow construction.

1. Connection Validation

Before any workflow is executed, the AI engine checks how tools are connected on your canvas. It ensures that:

• The output of one tool matches the input type of the next tool.
• No incompatible file types or disconnected nodes exist.
• Data dependencies are logically consistent across all steps.

This real-time feedback helps users identify and fix issues instantly, reducing trial-and-error and improving the accuracy of the final pipeline.

2. Pre-Execution Warnings

Before generating the final workflow code, GenXflo performs an AI-powered pre-execution check. This process identifies:

• Missing reference files or inputs
• Misconfigured parameters or incompatible file formats

The system highlights potential issues before export, preventing failed executions and wasted computational resources.

Once your workflow is designed and validated, the final step is to export it into executable code, transforming your visual design into a ready-to-run workflow package.

Simply click "Submit" on the GenXflo canvas to begin. A pop-up will appear confirming that your pipeline zip file is being generated. This package contains everything needed to reproduce your analysis, including workflow code, configuration files, and metadata.

Track Progress on the Dashboard

After submission, click "Go to Dashboard" to monitor the progress of your pipeline creation. Each workflow you submit appears here with one of four status indicators:

Submitted: The request has been received and queued for processing.
In Progress: The system is actively generating your pipeline files.
Succeeded: The workflow has been created successfully and is ready for download.
Failed: The pipeline creation didn't complete; you can download a log file for details.

This dashboard makes it easy to see exactly where your workflow stands and ensures complete transparency in the export process.

Visualize with "View Schema"

Once your pipeline is ready, you can click "View Schema" to see a detailed visual diagram of your workflow. This diagram shows how tools are connected, the data flow between steps, and the overall execution order.

Inside the schema popup, you'll find a "Download Schema Diagram" button, allowing you to save this visual representation to your local device for documentation or sharing.

Download Your Workflow

When the status changes to Succeeded, you can click "Download file" to retrieve your complete pipeline zip file. Before downloading, GenXflo asks for confirmation, giving you the option to proceed or cancel.

The downloaded zip file includes:

A workflow script (e.g., main.nf; Snakefile or workflow.wdl (in the future))
A configuration file (e.g., nextflow.config, snakemake.config (in the future))
Supporting metadata for reproducibility

You can now run the workflow on your preferred platform (Nextflow, and in the future Snakemake or WDL), share it with collaborators, or archive it for future use.

These files are fully executable on any system that supports the respective workflow language — from local machines to HPC clusters to cloud platforms.

Why exporting matters

Exporting your workflow gives you:

Reproducibility: Every parameter, input, and tool is encoded and documented.
Portability: Run your workflow anywhere, locally or in the cloud.
Transparency: Review and share your workflow's logic as open, human-readable code.
Collaboration: Send your pipeline to colleagues or publish it alongside your research.

GenXflo's export feature transforms your visual design into production-ready computational pipelines, seamlessly bridging the gap between graphical simplicity and computational rigor.

Adding Custom Components

One of GenXflo’s most powerful features is the ability to add your own custom bioinformatics tools, extending the platform far beyond its built-in library. The “Custom Components” feature allows users to integrate tools that aren’t yet available in the default list, making every workflow truly personalized and adaptable to evolving research needs.

With this option, you can bring your preferred command-line programs, scripts, or containers directly into the GenXflo environment and use them just like any other built-in component on the canvas.

Steps to Create Custom Components

Step 1: Create a New Pipeline

Start by clicking “Create New Pipeline.” You’ll be redirected to a setup page where you can enter some basic information:

• Pipeline Name – a unique name to identify your project.
• Description – a short overview of what this pipeline will do.

Once you’ve verified the details, click “Next” to move to the main workspace where you’ll design your workflow.

Step 2: Add a Custom Component

On the left sidebar of the workspace, select the Custom Components icon. Click the “Add Tool” button to open the configuration form for your new component.

This form lets you specify how GenXflo should locate and install your tool. You have three options:

• BioArch Repository – select this if your tool is available in the BioArch collection.
• Bioconda Repository – ideal for tools distributed via the Bioconda ecosystem.
• Other – For tools not in Bioconda or BioArch, either provide a Source code link (to build a container with AI) or paste the Custom Docker Image URL if you already have one

You can also use “Auto-Fill with AI” to automatically complete the tool configuration fields. Alternatively, you can manually enter the details if you prefer full control.

If your tool doesn’t yet have a Docker container, GenXflo can help — simply click “Generate Dockerfile with AI” to automatically create one. This ensures your tool is fully portable and reproducible across environments.

Once all fields are filled and confirmed, click “Add” to include your tool in the GenXflo library.

Step 3: Use and Manage Custom Tools

Your newly added component will now appear in the Custom Components section of the sidebar. You can drag and drop it onto the canvas just like any other tool and connect it within your workflow.

If you need to make changes later, click “Edit” above the component to reopen its configuration form. After making your updates, save the changes by clicking “Update.”

To remove a custom component, simply use the Delete button, giving you full flexibility over your custom tool library.

Why Choose GenXflo?

GenXflo is designed for research teams that value efficiency, reproducibility, and simplicity. It empowers both computational biologists and lab scientists to focus on science while automating the technical complexities of workflow management.

Key Features

Low-code, high impact

Build pipelines visually without learning complex workflow syntax, saving time and reducing errors.

Smart validation engine

Automatic AI-driven checks ensure workflows comply with WML best practices and data compatibility standards.

Reusable templates

Save workflows as templates for future experiments or share with collaborators to maintain consistency.

Integration ready

Exported pipelines run seamlessly on local machines or HPC clusters, supporting a variety of deployment options.

Reproducibility by design

All exported workflows include environment metadata, tool versions, and parameter definitions for transparent and reproducible analyses.

Custom component addition

Easily add custom components that are not included by default, expanding your workflow to incorporate a wide range of bioinformatics tools.

Powering Modern Bioinformatics Workflows

GenXflo supports a wide variety of research applications, making it a versatile solution for multiple fields of bioinformatics:

Whole Genome Sequencing

Align, call variants, and annotate genomes using integrated tools.

RNA-seq Analysis

Automate quality control, mapping, quantification, and differential expression analyses.

Metagenomics

Build scalable pipelines for taxonomic classification, abundance profiling, and diversity analyses.

Pre-PCR Workflows

Design and optimize primers efficiently with automated pre-PCR workflows to ensure accurate and reliable amplification.

Custom Workflows

Combine any supported tools to create unique workflows tailored to specific research needs.